West DW, Burd NA, Coffey VG, Baker SK, Burke LM,
Hawley JA, Moore DR, Stellingwerff T, Phillips SM. Rapid aminoacidemia enhances
myofibrillar protein synthesis and anabolic intramuscular
signaling responses after resistance exercise. Am J Clin Nutr. 2011
Sep;94(3):795-803.
Abstract
BACKGROUND:
Ingestion of whey or casein yields divergent patterns of
aminoacidemia that influence whole-body and skeletal muscle
myofibrillar protein synthesis (MPS) after exercise. Direct
comparisons of the effects of contrasting absorption rates
exhibited by these proteins are confounded by their differing
amino acid contents.
OBJECTIVE:
Our objective was to determine the effect of divergent
aminoacidemia by manipulating ingestion patterns of whey protein
alone on MPS and anabolic signaling after resistance exercise.
DESIGN:
In separate trials, 8 healthy men consumed whey protein either
as a single bolus (BOLUS; 25-g dose) or as repeated, small,
"pulsed" drinks (PULSE; ten 2.5-g drinks every 20 min) to mimic
a more slowly digested protein. MPS and phosphorylation of
signaling proteins involved in protein synthesis were measured
at rest and after resistance exercise.
RESULTS:
BOLUS increased blood essential amino acid (EAA) concentrations
above those of PULSE (162% compared with 53%, P < 0.001) 60
min after exercise, whereas PULSE resulted in a smaller but
sustained increase in aminoacidemia that remained elevated above
BOLUS amounts later (180-220 min after exercise, P < 0.05).
Despite an identical net area under the EAA curve, MPS was
elevated to a greater extent after BOLUS than after PULSE early
(1-3 h: 95% compared with 42%) and later (3-5 h: 193% compared
with 121%) (both P < 0.05). There were greater changes in the
phosphorylation of the Akt-mammalian target of rapamycin pathway
after BOLUS than after PULSE.
CONCLUSIONS:
Rapid aminoacidemia in the postexercise period enhances MPS and
anabolic signaling to a greater extent than an identical amount
of protein fed in small pulses that mimic a more slowly digested
protein. A pronounced peak aminoacidemia after exercise enhances
protein synthesis.
This trial was registered at clinicaltrials.gov as NCT01319513.
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Some believe that by ingesting small amounts of whey protein or
pulsing protein after we train you will get much growth than just
one big protein shake after we train. This intermittent feeding,
in theory mimics a more slowly digested protein. This technique is
very popular among athletes who believe in intermittent fasting.
Researchers from McMaster University in Canada have discovered
that strength athletes benefit more from ingesting the whey in one
large portion than in several small ones. In their study 8 healthy
men trained their quadriceps with 8 sets of 10 reps on a leg
extension machine. Immediaely after training half of the subjects
were given 1 shake that contained 25g of whey protein (BOLUS) and
the other half were given smaller shakes of 2.5g of whey every 20
minutes (PULSE). Blood was measured in the subjects for 320
minutes (~5.5 hours)
The design for this experiment is shown in the graph below.
Figure1:
Throughout the 320 minutes that the researchers monitored the
subjects' blood . They found the same amounts of essential amino
acids [Figure 2] and leucine [figure 3] in both the 25g group and
the 10 X 2.5g group.
Figure 2:
Figure 3:
Over the 5 hour period of time one can easily observe that the 25g
group [BOLUS] had a peak in the essential amino acid and leucine
concentration an hour after intake. In the 10 X 2.5 g group
[PULSE] seems to be no peak as seen in Figure 4.
Figure 4:
The excessive amount of essential amino acids and leucine seemed
to also stimulate the production of muscle fibre protein
[Myofibrillar FSR] discovered from samples of muscle tissue from
the subjects as seem below.
Figure 5:
So what does this tell us? Obviously pulsing stimulates
myofibrillar FSR (muscle gowth) however when it comes to good,
better or best it seems that a 1 time feeding of whey protein is
best. During that 1st hour post exercise it is probably a good
idea that the body gets as much EAA and leucine as possible to
maximally stimulate myofibrillar protein synthesis. The rapid rise
in extracellular essential amino acid concentrations, or possibly
of leucine alone, that occurred after BOLUS appears to underpin
the greater signal activation and protein synthetic response that
are observed after an acute bout of resistance exercise. I would
personally like to see a comparison of 25g to maybe 50g
immediately after training. I have a feeling slightly more might
even stimulate myofibrillar protein synthesis even further.
Anyway, this is a very interesting study.